Tight oil reservoirs are buried deeply, its permeability is low, and it is easily influenced by sensitivity. So, it is important to study the sensitivity of tight oil reservoir to prevent reservoir damage and improve oilfield efficient development. However, due to the difficulty of water injection in tight reservoirs, the flow velocity is slow and takes longer time. In this paper, an evaluation method of reservoir sensitivity flow experiment was adopted, and sensitivity experiment on some rock samples of four wells in Xinjiang Jimsar basin Formation tight oil was conducted. The results show that the average damage of water sensitivity is 43.64%, velocity sensitive damage is 24.3%, salt sensitivity damage is 33.80%, acid sensitivity damage is 15.66%,and the critical velocity is 24.63 m/d. This tight oil reservoir has medium water sensitivity and salt sensitivity while weak acid sensitivity and velocity sensitivity. Base on the research above we have a certain understanding of this reservoir's sensitivity, which provides a theoretical basis for rational exploitation of the oilfield and reduces the damage of tight reservoir.